We further performed qRT-PCR in necrotic bone tissue samples to confirm the expression of potential miRNAs. Our results showed that miR-127, miR-628-3p, and miR-1 were downregulated (P<0.05), and miR-885-5p, miR-483-3p, and miR-483-5p were upregulated (P<0.05). The expression of these miRNAs maintained the consistent tendency in bone as in serum. However, no significant difference in miR-432-5p expression was observed in necrotic bone tissue samples compared with that in serum. Hence, our results revealed a close relationship between miRNAs and AIONFH. Thus, we focused on these six miRNAs for further analysis in the following experiments.