List of number of microsatellite repeats for fungus specific locus in stands of Cetraria aculeata, Usnea antarctica and U. aurantiacoatra, supplement to: Lagostina, Elisa; Dal Grande, Francesco; Scur, M; Lorenz, Andrea; Andreev, M; Ruprecht, U; Søchting, U; Sancho, L G; Wirtz, Nora; Rozzi, R; Printzen, Christian (in prep.): Genetic structure and gene flow of three lichens forming fungi in the Maritime Antarctic and southern South America. Journal of Biogeography

DOI

Eight consistently amplifying markers were used for Cetraria aculeata. Samples of Usnea aurantiacoatra and U. antarctica were genotyped using 21 and 22 microsatellites markers, respectively. Detailed information on primers and PCR amplification can be found in Lutsak et al. (2016) and Lagostina et al. (2017). PCR amplicons were electrophoresed using an Applied Biosystems 3730 sequencer, with the LIZ 500 (C. aculeata)or LIZ 600 (Usnea sp.) size standards (Applied Biosystems, Waltham, Mass., USA). Allele sizes were manually scored using the Geneious 10 microsatellites tool (Kearse et al. 2012). Those SSR markers were used to study the effects of dispersal strategy and phylogeographic history on the population genetic structure of Antarctic lichens.

Identifier
DOI https://doi.org/10.1594/PANGAEA.906884
Metadata Access https://ws.pangaea.de/oai/provider?verb=GetRecord&metadataPrefix=datacite4&identifier=oai:pangaea.de:doi:10.1594/PANGAEA.906884
Provenance
Creator Lagostina, Elisa
Publisher PANGAEA - Data Publisher for Earth & Environmental Science
Publication Year 2019
Funding Reference German Science Foundation
Rights Creative Commons Attribution 4.0 International
OpenAccess true
Representation
Language English
Resource Type Supplementary Collection of Datasets
Format application/zip
Size 3 datasets
Discipline Earth System Research
Spatial Coverage (-73.257W, -64.095S, -55.360E, -50.636N)
Temporal Coverage Begin 2002-02-01T00:00:00Z
Temporal Coverage End 2018-03-08T00:00:00Z