The morphological response of Emiliania huxleyi to seawater carbonate chemistry changes: an inter-strain comparison, supplement to: Langer, Gerald; Probert, Ian; Nehrke, Gernot; Ziveri, Patrizia (2011): The morphological response of Emiliania huxleyi to seawater carbonate chemistry changes: an inter-strain comparison. Journal of Nannoplankton Research, 32(1), 29-34

Four strains of the coccolithophore Emiliania huxleyi (RCC1212, RCC1216, RCC1238, RCC1256) were grown in dilute batch culture at four CO2 levels ranging from ~200 µatm to ~1200 µatm. Coccolith morphology was analyzed based on scanning electron micrographs. Three of the four strains did not exhibit a change in morphology over the CO2 range tested. One strain (RCC1256) displayed an increase in the percentage of malformed coccoliths with increasing CO2 concentration. We conclude that the sensitivity of the coccolith-shaping machinery to carbonate chemistry changes is strain-specific. Although it has been shown before that carbonate chemistry related changes in growth- and calcification rate are strain-specific, there seems to be no consistent correlation between coccolith morphology and growth or calcification rate. We did not observe an increase in the percentage of incomplete coccoliths in RCC1256, indicating that the coccolith-shaping machinery per se is affected by acidification and not the signalling pathway that produces the stop-signal for coccolith growth.

In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Lavigne and Gattuso, 2011) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation by seacarb is 2014-01-20.

Identifier
DOI https://doi.org/10.1594/PANGAEA.826456
PID http://hdl.handle.net/10013/epic.37875.d001
Related Identifier https://cran.r-project.org/package=seacarb
Metadata Access https://ws.pangaea.de/oai/provider?verb=GetRecord&metadataPrefix=datacite4&identifier=oai:pangaea.de:doi:10.1594/PANGAEA.826456
Provenance
Creator Langer, Gerald; Probert, Ian; Nehrke, Gernot; Ziveri, Patrizia
Publisher PANGAEA - Data Publisher for Earth & Environmental Science
Contributor Yang, Yan
Publication Year 2011
Funding Reference Seventh Framework Programme
Rights Creative Commons Attribution 3.0 Unported
OpenAccess true
Representation
Language English
Resource Type Supplementary Dataset
Format text/tab-separated-values
Size 496 data points
Discipline Earth System Research
Spatial Coverage (-20.333W, -42.300S, 169.833E, 63.400N)
Temporal Coverage Begin 1998-09-01T00:00:00Z
Temporal Coverage End 2005-11-30T00:00:00Z